Tools of Recombinant DNA Technology
Tools of Recombinant DNA Technology: Overview
This Topic covers sub-topics such as Gel Electrophoresis, Restriction Enzymes, Cloning Vectors, Electroporation, Microinjection, Plasmid as Vector, Palindromic Nucleotide Sequence, Selectable Marker, Gene Gun and, Tools of Recombinant DNA Technology
Important Questions on Tools of Recombinant DNA Technology
A recombinant DNA is formed when sticky ends of vector DNA and foreign DNA join. The sticky ends are formed by which of the following enzymes:
Agrobacterium mediated genetic transformation described as natured genetic engineering is used to resist plants from:
Which of the following technique is used for separating the fragments of DNA cut by restriction endonucleases?
Biotechnologists refer to which of the following bacteria as a natural genetic engineer of plants.
Which of the following feature of a vector is required to identify the transformed cell?
In recombinant DNA technology, ‘molecular scissors’ are:
Which of the following compound is used in visualisation of DNA fragments in gel electrophoresis?
An extra-chromosomal, self-replicating part of the cell that has proven to be a boon to biotechnology is:
Read the following statements and choose the correct option:
i. Agrobacterium tumefaciens, a pathogen of several monocot plants, is able to deliver a piece of DNA known as "T-DNA".
ii. The tumor inducing (Ti) plasmid of Agrobacterium tumefaciens has been modified into a cloning vector, which is no more pathogenic to the plants.
The genes (a) ori, (b) ampR and (c) rop are associated with which of the following vectors:
Which of the following microorganism is best suited as a cloning vector to introduce nematode specific genes in plants?
Fragments of DNA segments can be separated by:
Restriction endonucleases are used as:
In genetic engineering, which of the following is used?
Identify the wrong statement with regard to Restriction Enzymes.
In gel electrophoresis, separated DNA fragments can be visualized with the help of
Disarmed tumour-inducing plasmid transforms:
Which of the following method is used to directly inject rDNA into the nucleus of an animal cell?
Complete the analogy and select the correct option.
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How many unpaired nucleotides are present in the sticky end of DNA fragment formed by EcoRI?
